Collect. Czech. Chem. Commun.
1979, 44, 3177-3182
https://doi.org/10.1135/cccc19793177
Activation of latent collagenase from polymorphonuclear leukocytes by cathepsin G
Mária Stančíková and Karel Trnavský
Research Institute of Rheumatic Diseases, 921 01 Piestany
Abstract
Cathepsin G was isolated from human polymorphonuclear leukocytes and purified by affinity chromatography on Antilysin-Sepharose column. Purified enzyme activated later collagenase isolated from leukocytes. Activation at 36°C was maximal after 30 min incubation. Inhibitors of cathepsin G - soya-bean trypsin inhibitor, diisopropyl phosphofluoridate and Antilysin were active in inhibiting the activation of latent collagenase by cathepsin G.
