Collect. Czech. Chem. Commun. 1979, 44, 3177-3182

Activation of latent collagenase from polymorphonuclear leukocytes by cathepsin G

Mária Stančíková and Karel Trnavský

Research Institute of Rheumatic Diseases, 921 01 Piestany


Cathepsin G was isolated from human polymorphonuclear leukocytes and purified by affinity chromatography on Antilysin-Sepharose column. Purified enzyme activated later collagenase isolated from leukocytes. Activation at 36°C was maximal after 30 min incubation. Inhibitors of cathepsin G - soya-bean trypsin inhibitor, diisopropyl phosphofluoridate and Antilysin were active in inhibiting the activation of latent collagenase by cathepsin G.