Collect. Czech. Chem. Commun. 1979, 44, 145-147

Hog pancreatic α-amylase; N-terminal sequence analysis

Ivan Kluh

Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6


Both isozymes of hog pancreatic amylase were digested with chymotrypsin, subtilisin and pronase. These digests were passed over a column of sulfoethyl-Sephadex C-25 and peptides not containing free NH2-groups thus isolated. It was shown that both isozymes have the same N-terminal amino acid sequence, pyrrolidonecarboxylic acid - tyrosine. No peptides with acetylated amino groups were found.